Best 25 Spliceosomal Deahbox Atpases Remodel Premrna To Activate Alternative Splice Sites

Spliceosomal Deahbox Atpases Remodel Premrna To Activate Alternative Splice Sites

How do DEAH-box ATPases help spliceosome search for alternative splice sites?

At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. Here, using budding yeast, we show that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3′ splice sites.

How does the spliceosome select splice sites for intron excision?

During pre-mRNA splicing, a central step in the expression and regulation of eukaryotic genes, the spliceosome selects splice sites for intron excision and exon ligation. In doing so, the spliceosome must distinguish optimal from suboptimal splice sites.

How is pre-mRNA splicing catalyzed?

Pre-mRNA splicing is catalyzed by the spliceosome, a dynamic ribonucleoprotein (RNP) machine comprising over eighty conserved proteins and five small nuclear RNAs (snRNAs) ( Wahl et al., 2009 ).

Does rprp22-k512a block rejection of a 3′ splice site?

However, in the presence of dominant-negative, ATPase-defective rPrp22-K512A, which blocks rejection of a 3′ splice site ( Mayas et al., 2006 ), exon ligation occurred exclusively at the upstream 3′ splice site ( Figure 4 B, lane 3).

Spliceosomal Deahbox Atpases Remodel Premrna To Activate Alternative Splice Sites

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